32.1.263 TwoSiteTotal

1 Function
2 Brief Description
3 Sample Curve
4 Parameters
5 Derived Parameters
6 Script Access
7 Function File
8 Category

Function

\[y = Bg + NS\cdot x + \frac{B_{max1}\cdot x}{K_1+x} + \frac{B_{max2}\cdot x}{K_2+x}\]

Brief Description

Two sites total binding function.

Sample Curve

Parameters

Number: 6

Names: Bg, NS, Bmax1, Bmax2, K1, K2

Meanings: Bg=y value at x is 0, NS=slope, Bmax1=first top asymptote, Bmax2=second top asymptote, K1=first equilibrium dissociation constant, K2 =second equilibrium dissociation constant

Lower Bounds: K1>0, K2>0

Upper Bounds: none

Derived Parameters

AffinityRatio=K2/K1

PercentHighAffinity=100*Bmax1/(Bmax1+Bmax2)

Script Access

nlf_TwoSiteTotal(x,Bg,NS,Bmax1,Bmax2,K1,K2)

Function File

TwoSiteTotal.fdf