32.1.223 ThermDena

Function

Upper baseline: \(r_b=r_i+r_sx\)

Lower baseline: \(l_b=l_i+l_sx\)

\[\Delta G = \Delta H \left( \frac{T_m-x}{T_m+273}\right)- \Delta C_p(T_m-x)+(x+273) \Delta C_p ln \left(\frac{T_m+273}{x+273} \right)\]

\[K=e^{\frac{\Delta G}{RT}}\]

\[y=\frac{K}{1+K}\left(u_b-l_b \right) + l_b\]

Brief Description

Thermal denaturation function

Sample Curve

Parameters

Number: 7

Names: ri, rs, li, ls, Tm, dh, dcp

Meanings: upper baseline intercept, upper baseline slope, lower baseline intercept, lower baseline slope, denaturant dependence of g, delta G (free energy) of folding

Lower Bounds: Tm > 0, dh > 0, dcp > 0

Upper Bounds: none

Script Access

nlf_ThermDena(x,ri,rs,li,ls,Tm,dh,dcp)

Function File

ThermDena.fdf