32.1.182 OneSiteTotalDepletion

1 Function
2 Brief Description
3 Sample Curve
4 Parameters
5 Script Access
6 Function File
7 Category

Function

\(y = \frac{-b+\sqrt{b^2-4ac}}{2a}, where \)
\(a = -NS-1\)
\(KdCPM = 1000 \times V \times KdnM \times SA\)
\(b = KdCPM+B_{max}+NS\times KdCPM + x+2\times NS\times x \)
\(c = -x(B_{max}+NS\times KdCPM + NS\times x)\)

Brief Description

Fit total binding accounting for ligand depletion.

Sample Curve

Parameters

Number: 5

Names: V, SA, KdnM, NS, Bmax

Meanings: V=reaction volume, SA=specific radioactivity, KdnM=equilibrium dissociation constant, NS=slope of nonspecific binding, Bmax=maximum specific binding

Lower Bounds: NS>0

Upper Bounds: none

Script Access

nlf_OneSiteTotalDepletion(x,V,SA,KdnM,NS,Bmax)

Function File

OneSiteTotalDepletion.fdf