32.1.180 OneSiteLigandDepletion
Contents
Function
\[R=NS+1\]
\[a=-R\]
\[KdCPM=1000\cdot SpAct\cdot vol\cdot KdNM\]
\[S=Hot+KdCPM\cdot \left(10^{(x-logKi)}+1\right)\]
\[b=Bmax+NS\cdot Hot+R\cdot S\]
\[c=-Hot\cdot (Bmax+S\cdot NS)\]
\[y=\frac{-b+\sqrt{b^{2}-4ac}}{2a}\]
Brief Description
One site competition binding accounting for radioligand depletion.
Sample Curve
Parameters
Number: 7
Names: logKi, Bmax, NS, Hot, KdNM, SpAct, Vol
Meanings: logKi= log10 Ki, Bma=top asymptote, NS=nonspecific binding fraction, Hot=radioligand, KdN=hot Kd, SpAct=specific radioadivity, Vol=reaction volume
Lower Bounds: none
Upper Bounds: none
Derived Parameters
Ki=10^(logKi)
Script Access
nlf_OneSiteLigandDepletion(x,logKi,Bmax,NS,Hot,KdNM,SpAct,Vol)
Function File
OneSiteLigandDepletion.fdf
Category
Binding