32.1.156 MixedInhibition2

Function

\[ V_{0}=\frac{V_{\max}}{\frac{K_{m}}{S}+1} \qquad K_{app}=\frac{K_{i}\left(\frac{s}{K_{m}}+1\right)}{1+\frac{s}{\alpha K_{m}}}\]

\[y=\frac{V_{0}}{2E}\left(E-I-K_{app}+\sqrt{\left(E-I-K_{app}\right)^{2}+4EK_{app}}\right)\]

Brief Description

Tight-binding mixed-model inhibition describing the kinetic response to substrate concentration.

Sample Curve

Parameters

Number: 6

Names: Vmax, Km, Ki, alpha, E, I

Meanings: none

Lower Bounds: Vmax > 0, Km > 0, Ki > 0, alpha > 0, E > 0 , I (none)

Upper Bounds: none

Script Access

nlf_MixedInhibition2(x,Vmax,Km,Ki,alpha,E,I)

Function File

MixedInhibition.fdf

Category

Enzyme Kinetics 2