32.1.4 AllostericModulator

1 Function
2 Brief Description
3 Sample Curve
4 Parameters
5 Derived Parameters
6 Script Access
7 Function File
8 Category

Function

\(alpha = 10^{logAlpha}\)

\(KbNM = 10^{logKb + 9}\)

\(HotOccupancy = \frac{RadioligandNM}{RadioligandNM + HotKDnm}\)

\(AlloNM = 10^{x + 9}\)

\(KAppNM = HotKDnm \cdot \left(\frac{\frac{AlloNM}{KBNM} + 1}{alpha \cdot \left(\frac{AlloNM}{KBNM}\right) + 1}\right)\)

\(y = \frac{y_{0} \cdot \left(\frac{RadioligandNM}{RadioligandNM + KAppNM}\right)}{HotOccupancy}\)

Brief Description

Allosteric modulator titration of radioligand binding based on the ternary complex model.

Sample Curve

Parameters

Number: 5

Names: logKb, logAlpha, y0, RadioligandNM, HotKdNM

Meanings: logKb,=log10 Kb, logAlpha=log10 ternary complex constant, y0=radioligand binding, RadioligandNM=radioligand concentration, HotKdNM=hot kd

Lower Bounds: none

Upper Bounds: none

Derived Parameters

Kb=10^(logKb)

Alpha=10^(logAlpha)

Script Access

nlf_AllostericModulator(x,logKb,logAlpha,y0,RadioligandNM,HotKdNM)

Function File

AllostericModulator.fdf